Denaturation of DNA
Definition : Denaturation is the transition from native to the denatured state of DNA.
Introduction : Denatured Macromolecule results by disrupted state, or coiled conformation while when same molecule present in ordered state/originally present molecular structure in nature called as native.
Denaturation is the transition from Native to Denatured state. When double stranded DNA or native DNA is heated the bonding forces between the two strands are weakened and the strands separate, thus denatured DAN is single stranded structure.
(normally denaturation accomplished by heating a DNA solution ).
Meeting Curve : A graph of varying property as a function of temperature.
Subtitle 1: The simplest way to detect denaturation in DNA, is to monitor the ability of DNA in solution to absorb ultraviolet light of 264 nm (nanometer) wavelength, as the bases of DNA (A260) absorb 260 nm light strongly
Note :
The amount of light absorbed by the nucleic acids is dependent on the structure of the molecule. The more ordered the molecular structure, the less light is absorbed, the phenomenon is called hypochromism. Therefore, free nucleotides absorb more light and thus a denatured nucleic acid (Denatured DNA) depicts phenomenon Hyberchromism.
Double stranded DNA A260 = 1.00
Single stranded DNA A260 = 1.37
Free bases (nucleotides) A260 = 1.60